An open source cryostage and software analysis method for detection of antifreeze activity.


The aim of this study is to provide the reader with a simple setup that can detect antifreeze proteins (AFP) by inhibition of ice recrystallisation in very small sample sizes. This includes an open source cryostage, a method for preparing and loading samples as well as a software analysis method. The entire setup was tested using hyperactive AFP from the… (More)
DOI: 10.1016/j.cryobiol.2016.03.010


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