An open letter to our readers on the use of antibodies

  title={An open letter to our readers on the use of antibodies},
  author={Clifford B. Saper},
  journal={Journal of Comparative Neurology},
  • C. Saper
  • Published 26 December 2005
  • Biology
  • Journal of Comparative Neurology
The use of immunohistochemistry has become ubiquitous in neuroscience. A large majority of papers now published in The Journal of Comparative Neurology use immunohistochemistry, and some papers may employ a battery of ten or more antibodies to examine issues of colocalization or cell typing. This pattern has resulted in a flood of new information. . . .but also a flood of misinformation. The Journal has repeatedly, over the last few years, received distressed communications from authors, who… 

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The aims of the present paper are to increase the awareness of the difficulties in proving the specificity of immunocytochemical labeling and to initiate a discussion on optimized standards.

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  • J. Fritschy
  • Biology
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A valuable tool for experienced researchers and beginners when planning or troubleshooting the immunofluorescence assay is added, outlining 10 current methods for improving sample processing in different stages of immun ofluorescence, including a section with further recommendations.

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Specificity Controls for Immunocytochemistry

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Comment from the Editor‐in‐Chief on correspondence in this issue on immuno‐techniques

  • J. McGrath
  • Biology
    British journal of pharmacology
  • 2011
In this issue of BJP the authors publish two letters commenting on the use of immunolabelling techniques, one concerned with Western Blots and the other with the need for inclusion of rigorous control data when reporting experiments employing immunotechniques.

Localization of a Wide-Ranging Panel of Antigens in the Rat Retina by Immunohistochemistry

Davidson’s fixative was less compatible with certain antibodies, such as the neurotransmitter γ-aminobutyric acid, the microglial marker iba1, the macroglial stress protein nestin, and the small heat shock proteins Hsp27 and αB-crystallin, shortfalls that somewhat temper enthusiasm concerning its use.

The effect of journal guidelines on the reporting of antibody validation

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