An o-toluidine method for body-fluid glucose determination.

@article{Dubowski2008AnOM,
  title={An o-toluidine method for body-fluid glucose determination.},
  author={Kurt M. Dubowski},
  journal={Clinical chemistry},
  year={2008},
  volume={54 11},
  pages={
          1919-20
        }
}
  • Kurt M. Dubowski
  • Published in Clinical chemistry 2008
  • Chemistry, Medicine
  • o-Toluidine, 6% (v/v) in glacial acetic acid, is used to determine glucose in biologic material after deproteinization with 3% (w/v) trichloracetic acid. A stable green color develops after heating at 100° for 10 min., and the absorbance is determined at 630 or 635 mµ. The reagent is stable for many months at room temperature, and the reaction follows Beer's Law over a very wide range of concentrations. The development of the procedure is discussed, as is the specificity of the method for… CONTINUE READING

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