An o-toluidine method for body-fluid glucose determination.

  title={An o-toluidine method for body-fluid glucose determination.},
  author={Kurt M. Dubowski},
  journal={Clinical chemistry},
o-Toluidine, 6% (v/v) in glacial acetic acid, is used to determine glucose in biologic material after deproteinization with 3% (w/v) trichloracetic acid. A stable green color develops after heating at 1000 for 10 mm., and the absorbance is determined at 630 or 635 mp. The reagent is stable for many months at room temperature, and the reaction follows Beer’s… CONTINUE READING