An iterative strategy for precursor ion selection for LC-MS/MS based shotgun proteomics.

@article{Zerck2009AnIS,
  title={An iterative strategy for precursor ion selection for LC-MS/MS based shotgun proteomics.},
  author={Alexandra Zerck and Eckhard Nordhoff and Anja Resemann and Ekaterina Mirgorodskaya and Detlef Suckau and Knut Reinert and Hans Lehrach and Johan Gobom},
  journal={Journal of proteome research},
  year={2009},
  volume={8 7},
  pages={3239-51}
}
Currently, the precursor ion selection strategies in LC-MS mainly choose the most prominent peptide signals for MS/MS analysis. Consequently, high-abundance proteins are identified by MS/MS of many peptides, whereas proteins of lower abundance might elude identification. We present a novel, iterative and result-driven approach for precursor ion selection that significantly increases the efficiency of an MS/MS analysis by decreasing data redundancy and analysis time. By simulating different… CONTINUE READING