An isotopic assay of dUTPase activity based on coupling with thymidylate synthase.

  title={An isotopic assay of dUTPase activity based on coupling with thymidylate synthase.},
  author={B Gołos and Wojciech Rode},
  journal={Acta biochimica Polonica},
  volume={46 3},
A new rapid, sensitive and convenient procedure is presented allowing determination of dUTPase activity. With [5-(3)H]dUTP used as the substrate, dUTPase, converts it to the corresponding monophosphate and is coupled with thymidylate synthase-catalyzed reaction, resulting in tritium release from [5-(3)H]dUMP. Following charcoal absorption of the labeled nuleotides, radioactivity of tritiated water is determined. The new assay was tested to show comparable results with a previously described… Expand
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