An isocratic separation of underivatized gentamicin components, 1H NMR assignment and protonation pattern.

Abstract

A simple method for the separation of the major components of commercial gentamicin sulfate (C-1, C-1a, C-2, C-2a) by high-performance liquid chromatography (HPLC) on an analytical and a semipreparative scale was developed. The method utilized ion-pair reversed-phase chromatography, isocratic elution with an aqueous solution containing 9% trifluoroacetic… (More)

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Cite this paper

@article{Lesniak2003AnIS, title={An isocratic separation of underivatized gentamicin components, 1H NMR assignment and protonation pattern.}, author={Wojciech G. Lesniak and John Mc Laren and Wesley R. Harris and Vincent L Pecoraro and Jochen Schacht}, journal={Carbohydrate research}, year={2003}, volume={338 24}, pages={2853-62} }