An isocratic high-performance liquid chromatography assay for CYP7A1-catalyzed cholesterol 7alpha-hydroxylation.

Abstract

A normal-phase, isocratic high-performance liquid chromatography assay is described for cholesterol 7alpha-hydroxylation catalyzed by CYP7A1, which corresponds to the first and rate-limiting step in the conversion of cholesterol into bile acids. This method is based on the conversion of the primary cytochrome P450 metabolite, 7alpha-hydroxycholesterol, into… (More)

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