An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA.

@article{Gill2000AnIO,
  title={An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA.},
  author={Peter Gill and Jonathan Whitaker and Christine Flaxman and Natasha Brown and John S. Buckleton},
  journal={Forensic science international},
  year={2000},
  volume={112 1},
  pages={
          17-40
        }
}
Validation of testing and interpretation protocols for low template DNA samples using AmpFlSTR Identifiler.
TLDR
Using the protocols and interpretation guidelines described here, LT-DNA testing is reliable and robust and implementation of this method, or one that is suitably verified, in conjunction with an appropriate quality control program ensures that LT- DNA testing is suitable for forensic purposes.
Validation and development of interpretation guidelines for low copy number (LCN) DNA profiling in New Zealand using the AmpFlSTR SGM Plus multiplex.
Efficacy and limits of genotyping low copy number (LCN) DNA samples by multiplex PCR of STR loci.
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This study resulted in the positive identification of a number of victims by comparing the LCN DNA profiles with the profiles from putative relatives and confirmed the data of recent other studies that a comprehensive interpretation strategy is dependent upon multiple replication of the PCR using the same extract together with the proper use of extraction and amplification controls.
SETTING INTERPRETATION THRESHOLDS AND RESULTS WITH LOW-LEVEL DNA ANALYSIS
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Next generation manufacturers’ kits are being made more sensitive with improved PCR master mixes and more robust DNA polymerases, which can potentially lead to labs pushing the envelope and getting results that may not represent the true DNA profile of the originating source due to stochastic effects including allele dropout or drop-in.
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