An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA.
@article{Gill2000AnIO, title={An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA.}, author={Peter Gill and Jonathan Whitaker and Christine Flaxman and Natasha Brown and John S. Buckleton}, journal={Forensic science international}, year={2000}, volume={112 1}, pages={ 17-40 } }
Figures and Tables from this paper
593 Citations
An investigation of the robustness of the consensus method of interpreting low-template DNA profiles.
- BiologyForensic science international. Genetics
- 2011
Validation of testing and interpretation protocols for low template DNA samples using AmpFlSTR Identifiler.
- BiologyCroatian medical journal
- 2009
Using the protocols and interpretation guidelines described here, LT-DNA testing is reliable and robust and implementation of this method, or one that is suitably verified, in conjunction with an appropriate quality control program ensures that LT- DNA testing is suitable for forensic purposes.
Validation and development of interpretation guidelines for low copy number (LCN) DNA profiling in New Zealand using the AmpFlSTR SGM Plus multiplex.
- BiologyForensic science international. Genetics
- 2010
Efficacy and limits of genotyping low copy number (LCN) DNA samples by multiplex PCR of STR loci.
- BiologyJournal de la Societe de biologie
- 2003
This study resulted in the positive identification of a number of victims by comparing the LCN DNA profiles with the profiles from putative relatives and confirmed the data of recent other studies that a comprehensive interpretation strategy is dependent upon multiple replication of the PCR using the same extract together with the proper use of extraction and amplification controls.
Efficacy and limits of genotyping low copy number DNA samples by multiplex PCR of STR loci
- Biology
- 2003
SETTING INTERPRETATION THRESHOLDS AND RESULTS WITH LOW-LEVEL DNA ANALYSIS
- Biology
- 2009
Next generation manufacturers’ kits are being made more sensitive with improved PCR master mixes and more robust DNA polymerases, which can potentially lead to labs pushing the envelope and getting results that may not represent the true DNA profile of the originating source due to stochastic effects including allele dropout or drop-in.
A comparison of the characteristics of profiles produced with the AMPFlSTR SGM Plus multiplex system for both standard and low copy number (LCN) STR DNA analysis.
- BiologyForensic science international
- 2001
Extended PCR conditions to reduce drop-out frequencies in low template STR typing including unequal mixtures.
- BiologyForensic science international. Genetics
- 2012
Stochastic sampling effects in STR typing: Implications for analysis and interpretation.
- BiologyForensic science international. Genetics
- 2014
Analysis of artificially degraded DNA using STRs and SNPs--results of a collaborative European (EDNAP) exercise.
- BiologyForensic science international
- 2006
References
SHOWING 1-10 OF 23 REFERENCES
Analysis and interpretation of mixed forensic stains using DNA STR profiling.
- BiologyForensic science international
- 1998
Development of guidelines to designate alleles using an STR multiplex system.
- Computer ScienceForensic science international
- 1997
The validation of a 7-locus multiplex STR test for use in forensic casework. (II), Artefacts, casework studies and success rates.
- BiologyInternational journal of legal medicine
- 1996
The performance of the SGM system with poor quality samples demonstrated its use as a rapid and powerful technique for individual identification as well as robust and reproducible under conditions consistent with those encountered in a forensic environment.
Validation of the applied biosystems Prism™ 377 automated sequencer for forensic short tandem repeat analysis
- BiologyElectrophoresis
- 1996
The Applied Biosystems (ABI) PrismTM 377 DNA sequencer has been evaluated in an attempt to increase the throughput of samples for short tandem repeat (STR) analysis, in both forensic casework and the…
Validation of the AMPFlSTR SGM plus system for use in forensic casework.
- BiologyForensic science international
- 2000
Statistical analyses to support forensic interpretation for a new ten-locus STR profiling system
- Environmental ScienceInternational Journal of Legal Medicine
- 2001
An investigation of match probability calculations is carried out and the consequent analyses are shown to provide support for proposed changes in how the FSS reports DNA results when very small match probabilities are involved.
Use of nested PCR to identify charred human remains and minute amounts of blood.
- BiologyJournal of forensic sciences
- 1998
The successful use of nested PCR for gender identification and reverse paternity testing in a forensic case where the only available materials consisted of charred human remains and a minute quantity of blood that were unsuitable for standard PCR.
A guide to interpreting single locus profiles of DNA mixtures in forensic cases.
- PhysicsJournal - Forensic Science Society
- 1991
Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.
- BiologyBioTechniques
- 1991
The extraction of DNA from semen and very small bloodstains using Chelex 100 is as efficient or more efficient than using proteinase K and phenol-chloroform extraction and DNA extracted from bloodstain seems less prone to contain PCR inhibitors when prepared by this method.
Preimplantation single-cell analysis of multiple genetic loci by whole-genome amplification.
- BiologyProceedings of the National Academy of Sciences of the United States of America
- 1994
Preamplification has now been applied successfully to the analysis of the two major mutations responsible for Tay-Sachs disease and of a common restriction polymorphism in the gene responsible for hemophilia A.