An investigation of intracellular glycosylation activities in CHO cells: Effects of nucleotide sugar precursor feeding

@article{Wong2010AnIO,
  title={An investigation of intracellular glycosylation activities in CHO cells: Effects of nucleotide sugar precursor feeding},
  author={Niki S.C. Wong and Lydia Wati and Peter Morin Nissom and Hua-tao Feng and M.M. Lee and Miranda Gek Sim Yap},
  journal={Biotechnology and Bioengineering},
  year={2010},
  volume={107}
}
Controlling glycosylation of recombinant proteins produced by CHO cells is highly desired as it can be directed towards maintaining or increasing product quality. To further our understanding of the different factors influencing glycosylation, a glycosylation sub‐array of 79 genes and a capillary electrophoresis method which simultaneously analyzes 12 nucleotides and 7 nucleotide sugars; were used to generate intracellular N‐glycosylation profiles. Specifically, the effects of nucleotide sugar… Expand
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