An inverse relationship between KAI1 expression, invasive ability, and MMP-2 expression and activity in bladder cancer cell lines.

@article{You2012AnIR,
  title={An inverse relationship between KAI1 expression, invasive ability, and MMP-2 expression and activity in bladder cancer cell lines.},
  author={Jingjing You and Michele C. Madigan and Alexandra J. Rowe and Mila Sajinovic and P. J. Russell and Paul Jackson},
  journal={Urologic oncology},
  year={2012},
  volume={30 4},
  pages={502-8}
}
OBJECTIVE To investigate the relationship between the expression of the cancer metastasis suppressor gene KAI1 and MMP-2 and MMP-9 in human bladder cancer cell lines that express variable levels of KAI1. MATERIALS AND METHODS Five bladder cancer cell lines (BL-28/0, BL-13/0, BL-17/0/×1, B10, and D2) were grown in standard culture conditions. Gelatinase activities in serum-free conditioned medium were assessed using gelatin zymography. Whole cell lysates were prepared and Western blotting used… CONTINUE READING

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