An internal standard method for the unattended high-performance liquid chromatographic analysis of ascorbic acid in blood components.

@article{Kutnink1987AnIS,
  title={An internal standard method for the unattended high-performance liquid chromatographic analysis of ascorbic acid in blood components.},
  author={M A Kutnink and Wayne Chris Hawkes and E E Schaus and Stanley Teruo Omaye},
  journal={Analytical biochemistry},
  year={1987},
  volume={166 2},
  pages={424-30}
}
A paired-ion, reversed-phase, high-performance liquid chromatography procedure using electrochemical detection and internal standard quantitation based on isoascorbic acid (IA) is described for the determination of ascorbic acid (AA) in blood cells and plasma. By correcting for vial-to-vial variations in the AA oxidation rate, use of IA as an internal standard overcomes a major problem associated with AA instability and eliminates the necessity of assaying samples immediately after they are… CONTINUE READING