An internal control for routine diagnostic PCR: design, properties, and effect on clinical performance.

@article{Rosenstraus1998AnIC,
  title={An internal control for routine diagnostic PCR: design, properties, and effect on clinical performance.},
  author={Maurice Jay Rosenstraus and Zhenglong Wang and S. Y. Chang and D DeBonville and Joanne Spadoro},
  journal={Journal of clinical microbiology},
  year={1998},
  volume={36 1},
  pages={
          191-7
        }
}
We constructed internal controls (ICs) to provide assurance that clinical specimens are successfully amplified and detected. The IC nucleic acids contain primer binding regions identical to those of the target sequence and contain a unique probe binding region that differentiates the IC from amplified target nucleic acid. Because only 20 copies of the IC are introduced into each test sample, a positive IC signal indicates that amplification was sufficient to generate a positive signal from… CONTINUE READING

Similar Papers

Citations

Publications citing this paper.
SHOWING 1-10 OF 91 CITATIONS, ESTIMATED 56% COVERAGE

FILTER CITATIONS BY YEAR

1998
2018

CITATION STATISTICS

  • 6 Highly Influenced Citations

  • Averaged 3 Citations per year over the last 3 years