An internal RNA element in the P3 cistron of Wheat streak mosaic virus revealed by synonymous mutations that affect both movement and replication.

@article{Choi2005AnIR,
  title={An internal RNA element in the P3 cistron of Wheat streak mosaic virus revealed by synonymous mutations that affect both movement and replication.},
  author={Il-Ryong Choi and Kempton M Horken and Drake C. Stenger and R. French},
  journal={The Journal of general virology},
  year={2005},
  volume={86 Pt 9},
  pages={2605-14}
}
Multiple synonymous substitution mutations in the Wheat streak mosaic virus P3 cistron did not affect translation in vitro but rendered the virus incapable of systemic infection. Multiple synonymous substitutions in the cylindrical inclusion cistron did not alter infectivity or in vitro translation. To assess replication and movement phenotypes, P3 mutations were placed in context with a GUS reporter gene. GUS activity measured in barley protoplasts 36 h post-transfection indicated that mutants… CONTINUE READING
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