An interference-free fluorescent assay of telomerase for the high-throughput analysis of inhibitors.

@article{Francis2003AnIF,
  title={An interference-free fluorescent assay of telomerase for the high-throughput analysis of inhibitors.},
  author={Rawle Francis and Simon H Friedman},
  journal={Analytical biochemistry},
  year={2003},
  volume={323 1},
  pages={65-73}
}
We have developed a telomerase assay that can quickly and accurately rank the ability of molecules to inhibit telomerase activity. It is based on the method of Orlando and co-workers which utilizes PicoGreen to detect dsDNA formed during the polymerase chain reaction (PCR) amplification of telomerase products. PCR cycles were optimized to give as linear a… CONTINUE READING