An inter-laboratory ring trial for the detection and isolation of Mycobacterium avium subsp. paratuberculosis from raw milk artificially contaminated with naturally infected faeces.

@article{Donaghy2008AnIR,
  title={An inter-laboratory ring trial for the detection and isolation of Mycobacterium avium subsp. paratuberculosis from raw milk artificially contaminated with naturally infected faeces.},
  author={J. A. Donaghy and Michael T. Rowe and Jan L W Rademaker and P. Hammer and Lieve Herman and Valerie De Jonghe and B{\'e}atrice Blanchard and Koenraad Duhem and Elena Vindel},
  journal={Food microbiology},
  year={2008},
  volume={25 1},
  pages={128-35}
}
There is a need for standardised, robust, reproducible molecular and culture methods to achieve clarification of the inactivation of Mycobacterium avium subsp. paratuberculosis (Map), the causative microbial agent of Johne's disease, in (faecally) contaminated milk and other food products such as meat. This study assessed the performance of a commercially available Map DNA extraction kit for milk Adiapure and accompanying PCR detection kit Adiavet alongside 'in-house' molecular and culture… CONTINUE READING

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