An intein-based genetic selection allows the construction of a high-quality library of binary patterned de novo protein sequences.

@article{Bradley2005AnIG,
  title={An intein-based genetic selection allows the construction of a high-quality library of binary patterned de novo protein sequences.},
  author={Luke H. Bradley and Ralph E Kleiner and Anna F Wang and Michael H Hecht and David P. Wood},
  journal={Protein engineering, design & selection : PEDS},
  year={2005},
  volume={18 4},
  pages={201-7}
}
Combinatorial libraries of synthetic DNA are increasingly being used to identify and evolve proteins with novel folds and functions. An effective strategy for maximizing the diversity of these libraries relies on the assembly of large genes from smaller fragments of synthetic DNA. To optimize library assembly and screening, it is desirable to remove from the synthetic libraries any sequences that contain unintended frameshifts or stop codons. Although genetic selection systems can be used to… CONTINUE READING
13 Citations
1 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 13 extracted citations

References

Publications referenced by this paper.

Edited by Andrew Griffiths Intein-based library selection

  • J. C. Spurlino, G. Y. Lu, F. A. Quiocho
  • Nat. Biotechnol.,
  • 1991
Highly Influential
9 Excerpts

Similar Papers

Loading similar papers…