An integrated strategy for identification and relative quantification of site-specific protein phosphorylation using liquid chromatography coupled to MS2/MS3.

@article{Wolschin2005AnIS,
  title={An integrated strategy for identification and relative quantification of site-specific protein phosphorylation using liquid chromatography coupled to MS2/MS3.},
  author={Florian Wolschin and Ute Lehmann and Mirko Glinski and Wolfram Weckwerth},
  journal={Rapid communications in mass spectrometry : RCM},
  year={2005},
  volume={19 24},
  pages={3626-32}
}
Reversible and differential multisite protein phosphorylation is an important mechanism controlling the activity of cellular proteins. Here we describe a robust and highly selective approach for the identification and relative quantification of site-specific phosphorylation events. This integrated strategy has three major parts: visualisation of phosphorylated proteins using fluorescently stained polyacrylamide gels, determination of the phosphorylation site(s) using automatic MS3 triggered by… CONTINUE READING

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