An integrated single- and two-photon non-diffracting light-sheet microscope.

  title={An integrated single- and two-photon non-diffracting light-sheet microscope.},
  author={Sze Cheung Lau and Hoi Chun Chiu and Luwei Zhao and Teng Zhao and Michael Ming-tak Loy and Shengwang Du},
  journal={The Review of scientific instruments},
  volume={89 4},
We describe a fluorescence optical microscope with both single-photon and two-photon non-diffracting light-sheet excitations for large volume imaging. With a special design to accommodate two different wavelength ranges (visible: 400-700 nm and near infrared: 800-1200 nm), we combine the line-Bessel sheet (LBS, for single-photon excitation) and the scanning Bessel beam (SBB, for two-photon excitation) light sheet together in a single microscope setup. For a transparent thin sample where the… 

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