An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets.

@article{Hufnagel2009AnIC,
  title={An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets.},
  author={Hansj{\"o}rg Hufnagel and Ansgar M Huebner and Carina G{\"u}lch and Katharina G{\"u}se and Chris Abell and Florian Hollfelder},
  journal={Lab on a chip},
  year={2009},
  volume={9 11},
  pages={
          1576-82
        }
}
We present a modular system of microfluidic PDMS devices designed to incorporate the steps necessary for cell biological assays based on mammalian tissue culture 'on-chip'. The methods described herein include the on-chip immobilization and culturing of cells as well as their manipulation by transfection. Assessment of cell viability by flow cytrometry suggests low attrition rates (<3%) and excellent growth properties in the device for up to 7 days for CHO-K1 cells. To demonstrate that key… CONTINUE READING
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