An integrated approach to extreme thermostabilization and affinity maturation of an antibody.

@article{McConnell2013AnIA,
  title={An integrated approach to extreme thermostabilization and affinity maturation of an antibody.},
  author={Audrey D. McConnell and Vladimir Spasojevich and John L. Macomber and Irina P Krapf and Andy P. Chen and Joseph C Sheffer and Ashley D. Berkebile and Robert A. Horlick and Steven Neben and David John King and Peter M. Bowers},
  journal={Protein engineering, design \& selection : PEDS},
  year={2013},
  volume={26 2},
  pages={
          151-64
        }
}
Antibodies are important tools for a broad range of applications due to their high specificity and ability to recognize virtually any target molecule. However, in order to be practically useful, antibodies must be highly stable and bind their target antigens with high affinity. We present a combinatorial approach to generate high-affinity, highly stable antibodies through the design of stable frameworks, specificity grafting and maturation via somatic hypermutation in vitro. By collectively… 
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