An integrated approach for finding overlooked genes in yeast

@article{Kumar2002AnIA,
  title={An integrated approach for finding overlooked genes in yeast},
  author={Anuj Kumar and P. Matthew Harrison and Kei-Hoi Cheung and Ning Lan and Nathaniel Echols and Paul Bertone and Perry Miller and Mark B Gerstein and Michael Snyder},
  journal={Nature Biotechnology},
  year={2002},
  volume={20},
  pages={58-63}
}
We report here the discovery of 137 previously unappreciated genes in yeast through a widely applicable and highly scalable approach integrating methods of gene-trapping, microarray-based expression analysis, and genome-wide homology searching. Our approach is a multistep process in which expressed sequences are first trapped using a modified transposon that produces protein fusions to β-galactosidase (β-gal); non-annotated open reading frames (ORFs) translated as β-gal chimeras are selected as… CONTINUE READING

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