An insulator blocks spreading of histone acetylation and interferes with RNA polymerase II transfer between an enhancer and gene.

@article{Zhao2004AnIB,
  title={An insulator blocks spreading of histone acetylation and interferes with RNA polymerase II transfer between an enhancer and gene.},
  author={Hui Ming Zhao and Ann Dean},
  journal={Nucleic acids research},
  year={2004},
  volume={32 16},
  pages={4903-19}
}
We studied the mechanism by which an insulator interrupts enhancer signaling to a gene using stably replicated chromatin templates containing the human beta-globin locus control region HS2 enhancer and a target globin gene. The chicken beta-globin 5' HS4 (cHS4) insulator acted as a positional enhancer blocker, inhibiting promoter remodeling and transcription activation only when placed between the enhancer and gene. Enhancer blocking by cHS4 reduced histone hyperacetylation across a zone… CONTINUE READING