An insertion sequence prepares Pseudomonas putida S12 for severe solvent stress.

@article{Wery2001AnIS,
  title={An insertion sequence prepares Pseudomonas putida S12 for severe solvent stress.},
  author={J Wery and Budi Juliman Hidayat and Jasper Kieboom and Jan A. M. de Bont},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 8},
  pages={5700-6}
}
The novel insertion sequence ISS12 plays a key role in the tolerance of Pseudomonas putida S12 to sudden toluene stress. Under normal culturing conditions the P. putida S12 genome contained seven copies of ISS12. However, a P. putida S12 population growing to high cell density after sudden addition of a separate phase of toluene carried eight copies. The survival frequency of cells in this variant P. putida S12 population was 1000 times higher than in "normal" P. putida S12 populations… CONTINUE READING
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