An inhibitory influence of granulosa cells and follicular fluid upon porcine oocyte meiosis in vitro.

@article{Tsafriri1975AnII,
  title={An inhibitory influence of granulosa cells and follicular fluid upon porcine oocyte meiosis in vitro.},
  author={Alex Tsafriri and Cornelia P. Channing},
  journal={Endocrinology},
  year={1975},
  volume={96 4},
  pages={922-7}
}
Isolated oocytes will resume meiosis spontaneously in vitro whereas follicle-enclosed oocytes will remain in the dictyate stage when cultured unless they have been exposed to gonadotropins in vivo or in vitro. To examine the source of the follicular inhibitory influence, porcine oocytes have been cultured alone, with hemisections of follicle wall, granulosa cells, or with follicular fluid. Oocytes isolated from medium-sized (3-5 min) follicles resumed meiosis when cultured; 77.5 plus or minus 3… CONTINUE READING

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