An inhibitor-free assay of acetylcholinesterase and butyrylcholinesterase in the cerebrospinal fluid.

@article{Kluge1999AnIA,
  title={An inhibitor-free assay of acetylcholinesterase and butyrylcholinesterase in the cerebrospinal fluid.},
  author={Harald H Kluge and Wolfram H Kluge and W. Hartmann},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1999},
  volume={282 1-2},
  pages={135-45}
}
An inhibitor-free assay for the simultaneous determination of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in the cerebrospinal fluid (CSF) is described. It is based on our finding that the individual activity ratios of BChE on both its substrates acetylthiocholine (ACh) and butyrylthiocholine (BCh) in the CSF and in the parallel serum are identical under conditions of at least 5 mmol/l substrate concentration (Q(BChE)SE = Q(BChe)CSF). Considering that AChE only reacts with ACh… CONTINUE READING