An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasion.

@article{Wang2010AnIL,
  title={An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasion.},
  author={Yi-Ting Wang and Chia-Feng Tsai and T Hong and Chih-Chiang Tsou and Pei-Yi Lin and Szu-Hua Pan and T S Hong and Pan-Chyr Yang and Ting-Yi Sung and Wen-Lian Hsu and Yu-Ju Chen},
  journal={Journal of proteome research},
  year={2010},
  volume={9 11},
  pages={5582-97}
}
Aberrant protein phosphorylation plays important roles in cancer-related cell signaling. With the goal of achieving multiplexed, comprehensive, and fully automated relative quantitation of site-specific phosphorylation, we present a simple label-free strategy combining an automated pH/acid-controlled IMAC procedure and informatics-assisted SEMI (sequence, elution time, mass-to-charge, and internal standard) algorithm. The SEMI strategy effectively increased the number of quantifiable peptides… CONTINUE READING