An inducible system for highly efficient production of recombinant adeno-associated virus (rAAV) vectors in insect Sf9 cells.

@article{Aslanidi2009AnIS,
  title={An inducible system for highly efficient production of recombinant adeno-associated virus (rAAV) vectors in insect Sf9 cells.},
  author={George V. Aslanidi and Kenneth Lamb and Sergei Zolotukhin},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2009},
  volume={106 13},
  pages={
          5059-64
        }
}
Production of clinical-grade gene therapy vectors for human trials remains a major hurdle in advancing cures for a number of otherwise incurable diseases. We describe a system based on a stably transformed insect cell lines harboring helper genes required for vector production. Integrated genes remain silent until the cell is infected with a single baculovirus expression vector (BEV). The induction of expression results from a combination of the amplification of integrated resident genes (up to… CONTINUE READING

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