An indirect enzyme-linked immunosorbent assay (ELISA) for the detection and quantitation of antisperm antibodies.

Several recent comparative investigations using various assays to detect and quantitate levels of antibody to human spermatozoa have produced widely varying results. In an attempt to reduce test variability, an indirect enzyme-linked immunosorbent assay (ELISA) was devised to measure antisperm antibodies. A standardized protocol was adapted employing sperm… (More)