An indirect anti-immunoglobulin rosetting reaction to detect alloantibodies to human lymphocytes.

@article{Bright1977AnIA,
  title={An indirect anti-immunoglobulin rosetting reaction to detect alloantibodies to human lymphocytes.},
  author={Susan Bright and Alastair J. Munro and Yvonne Lawson and Valerie C. Joysey and Rosemarie Coombs},
  journal={Journal of immunological methods},
  year={1977},
  volume={18 1-2},
  pages={55-62}
}
A test is reported which detects alloantibody, absorbed onto the surface of human lymphocytes from multiparous antisera, by means of a red cell rosette assay. The red cells, trypsinised ox, are coupled with anti-immunoglobulin using chromic chloride. The antiglobulin used is rabbit anti-human IgG (Fc), chosen to avoid reaction with the surface immunoglobulin naturally present on human B lymphocytes. The reaction is termed the Indirect Anti-immunoglobulin Rosetting Reaction (IARR). The IARR is… CONTINUE READING