An independently folding domain of RNA tertiary structure within the Tetrahymena ribozyme.

@article{Murphy1993AnIF,
  title={An independently folding domain of RNA tertiary structure within the Tetrahymena ribozyme.},
  author={Felicia L. Murphy and Thomas R Cech},
  journal={Biochemistry},
  year={1993},
  volume={32 20},
  pages={5291-300}
}
The Tetrahymena thermophila pre-rRNA contains a 413-nucleotide self-splicing group I intron. This intron has been converted into a sequence-specific endonuclease or ribozyme. A 160-nucleotide portion of the ribozyme consisting of both highly conserved sequence elements (P4 and P6) and nonconserved peripheral extensions (P5abc and P6ab) was synthesized as a separate molecule. Solvent-based Fe(II)-EDTA, a probe that monitors higher-order RNA structure, revealed a protection pattern that was a… CONTINUE READING

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