An inactive mutant of the alpha subunit of protein kinase CK2 that traps the regulatory CK2beta subunit.

@article{Cosmelli1997AnIM,
  title={An inactive mutant of the alpha subunit of protein kinase CK2 that traps the regulatory CK2beta subunit.},
  author={Diego Cosmelli and Marcelo Antonelli and Catherine C. Allende and Jorge E. Allende},
  journal={FEBS letters},
  year={1997},
  volume={410 2-3},
  pages={391-6}
}
Protein kinase CK2 (casein kinase 2) is a ubiquitous Ser/Thr protein kinase involved in cell proliferation. Mutation of the alpha subunit of the Xenopus laevis CK2 to change aspartic acid 156 to alanine (CK2alphaA156) resulted in an inactive enzyme. The CK2alphaA156 mutant, however, binds the regulatory subunit as measured by retention of beta on a nickel chelating column mediated by (His)6-tagged CK2alphaA156. Addition of CK2alphaA156 also caused beta to shift sedimentation in a sucrose… CONTINUE READING

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