An in vivo-In vitro study of the use of a human skin equivalent for irritancy screening of fatty acids.

@article{Boelsma1997AnIV,
  title={An in vivo-In vitro study of the use of a human skin equivalent for irritancy screening of fatty acids.},
  author={Esther Boelsma and H Tanojo and Harry E. Bodd{\'e} and Maria Ponec},
  journal={Toxicology in vitro : an international journal published in association with BIBRA},
  year={1997},
  volume={11 4},
  pages={365-76}
}
A human skin equivalent (HSE) consisting of reconstructed epidermis on a fibroblast-populated collagen gel was evaluated as a model for irritancy screening. The irritancy potential of a series of saturated and unsaturated fatty acids was investigated in vivo under short-term exposure conditions using transepidermal water loss (TEWL), laser Doppler velocimetry (LDV) and the penetration of hexyl nicotinate as parameters. The effects of the fatty acids in vitro were studied after topical… CONTINUE READING