An in vivo, luciferase-based, Agrobacterium-infiltration assay system: implications for post-transcriptional gene silencing

@article{Cazzonelli2006AnIV,
  title={An in vivo, luciferase-based, Agrobacterium-infiltration assay system: implications for post-transcriptional gene silencing},
  author={Christopher Ian Cazzonelli and Jeff P Velten},
  journal={Planta},
  year={2006},
  volume={224},
  pages={582-597}
}
An in vivo assay system for analyzing transient luciferase expression in tobacco leaves infused with Agrobacterium tumefaciens is described. The system makes use of A. tumefaciens harboring T-DNA vectors containing either an intron-containing firefly (Photinus pyralis) luciferase (EC 1.13.12.7) gene or an intron-containing sea pansy (Renilla reniformis) luciferase (EC 1.13.12.5) gene. Single or mixed Agrobacterium lines were infiltrated into leaf tissues (Nicotiana tabacum or Nicotiana… CONTINUE READING

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