An in vitro system for developmental and genetic studies of Leishmania donovani phosphoglycans.

@article{Goyard2003AnIV,
  title={An in vitro system for developmental and genetic studies of Leishmania donovani phosphoglycans.},
  author={Sophie Goyard and Hiroaki Segawa and Jennifer C Gordon and Melissa Showalter and R Duncan and Salvatore Joseph Turco and Stephen M Beverley},
  journal={Molecular and biochemical parasitology},
  year={2003},
  volume={130 1},
  pages={31-42}
}
Glycoconjugates have been shown to play important roles in Leishmania development. However, the ability to study these molecules and other processes would benefit greatly from improved methods for genetic manipulation and analysis of the amastigote stage. This is especially challenging for L. donovani, the agent of the most severe form of leishmaniasis, which can rapidly lose virulence during in vitro culture. Here we report on a clonal subline of an L. donovani 1S2D (LdBob or LdB), which… CONTINUE READING