An in vitro fluorescence based study of initiation of RNA synthesis by influenza B polymerase

@inproceedings{Reich2017AnIV,
  title={An in vitro fluorescence based study of initiation of RNA synthesis by influenza B polymerase},
  author={Stefan Reich and Delphine Guilligay and Stephen Cusack},
  booktitle={Nucleic acids research},
  year={2017}
}
Influenza polymerase replicates, via a complementary RNA intermediate (cRNA), and transcribes the eight viral RNA (vRNA) genome segments. To initiate RNA synthesis it is bound to the conserved 5΄ and 3΄ extremities of the vRNA or cRNA (the 'promoter'). 5΄-3΄ base-pairing in the distal promoter region is essential to position the template RNA at the polymerase active site, as shown by a new crystal structure with the 3΄ end threading through the template entry tunnel. We develop fluorescence… CONTINUE READING
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References

Publications referenced by this paper.
Showing 1-10 of 55 references

The role of the priming loop in RNA synthesis

  • A. J. Te Velthuis, N. C. Robb, A. N. Kapanidis, E. Fodor
  • Nat. Microbiol.,
  • 2016
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