An in vitro assay for Cdc20-dependent mitotic anaphase-promoting complex activity from budding yeast.

@article{Schuyler2009AnIV,
  title={An in vitro assay for Cdc20-dependent mitotic anaphase-promoting complex activity from budding yeast.},
  author={Scott C. Schuyler and Andrew W. Murray},
  journal={Methods in molecular biology},
  year={2009},
  volume={545},
  pages={
          271-85
        }
}
Cell cycle transitions are controlled, in part, by ubiquitin-dependent proteolysis. In mitosis, the metaphase to anaphase transition is governed by an E3 ubiquitin ligase called the cyclosome or Anaphase-Promoting Complex (APC), and a WD40-repeat protein co-factor called Cdc20. In vitro Cdc20-dependent APC (APC(Cdc20)) assays have been useful in the identification and validation of target substrates, and in the study of APC enzymology and regulation. Many aspects of the regulation of cell cycle… 
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Mps 1 Mph 1 Kinase Phosphorylates Mad 3 to Inhibit Cdc 20 Slp 1-APC / C and Maintain Spindle Checkpoint Arrests
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