An improved yeast transformation method for the generation of very large human antibody libraries.

@article{Benatuil2010AnIY,
  title={An improved yeast transformation method for the generation of very large human antibody libraries.},
  author={Lorenzo Benatuil and Jennifer M. Perez and Jonathan Belk and Chung-Ming Hsieh},
  journal={Protein engineering, design & selection : PEDS},
  year={2010},
  volume={23 4},
  pages={
          155-9
        }
}
Antibody library selection by yeast display technology is an efficient and highly sensitive method to identify binders to target antigens. This powerful selection tool, however, is often hampered by the typically modest size of yeast libraries (approximately 10(7)) due to the limited yeast transformation efficiency, and the full potential of the yeast display technology for antibody discovery and engineering can only be realized if it can be coupled with a mean to generate very large yeast… CONTINUE READING

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Improved yeast transformation method

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