An improved technique for observing both membranous organelles and cytoskeleton in saponin-extracted cells.

@article{Araki1993AnIT,
  title={An improved technique for observing both membranous organelles and cytoskeleton in saponin-extracted cells.},
  author={Naoko Araki and Tai Gyu Lee and Hirotsugu Matsubara and Y. Takashima},
  journal={Journal of electron microscopy},
  year={1993},
  volume={42 1},
  pages={
          51-4
        }
}
Detergent-extraction of soluble cytoplasmic proteins under low osmotic pressure induces severe morphological damage, particularly on membranous organelles, although this processing is often required to observe cytoskeletal elements in the cells. In the present study, the osmotic adjustment of the rinsing solution and fixative, which were used after the detergent-extraction using saponin, has greatly improved the ultrastructure of membranous organelles. This improved method allowed us to observe… CONTINUE READING