An improved technique for isolating codominant compound microsatellite markers

  title={An improved technique for isolating codominant compound microsatellite markers},
  author={Chunlan L. Lian and Md. Abdul Wadud and Qifang Geng and Kenichiro Shimatani and Taizo Hogetsu},
  journal={Journal of Plant Research},
An approach for developing codominant polymorphic markers (compound microsatellite (SSR) markers), with substantial time and cost savings, is introduced in this paper. In this technique, fragments flanked by a compound SSR sequence at one end were amplified from the constructed DNA library using compound SSR primer (AC)6(AG)5 or (TC)6(AC)5 and an adaptor primer for the suppression-PCR. A locus-specific primer was designed from the sequence flanking the compound SSR. The primer pairs of the… CONTINUE READING
Highly Cited
This paper has 144 citations. REVIEW CITATIONS

From This Paper

Figures, tables, and topics from this paper.


Publications citing this paper.
Showing 1-10 of 89 extracted citations

Microsatellite markers for the critically endangered elm species Ulmus gaussenii (Ulmaceae).

Genes & genetic systems • 2016
View 2 Excerpts
Highly Influenced

144 Citations

Citations per Year
Semantic Scholar estimates that this publication has 144 citations based on the available data.

See our FAQ for additional information.


Publications referenced by this paper.
Showing 1-10 of 11 references

GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism

M Raymond, F Rousset
J Hered • 1995
View 2 Excerpts
Highly Influenced

Development of microsatellite markers in black locust (Robinia pseudoacacia) using a dual-suppression-PCR technique

C Lian, T Hogetsu
Mol Ecol Notes • 2002
View 3 Excerpts

Integration of simple sequence repeat and markers into a soybean linkage map

MS Akkaya, RC Shoemaker, JE Specht, AA Bhagwat, PB Cregan
Crop Sci • 1995
View 3 Excerpts

Similar Papers

Loading similar papers…