An improved technique for isolating codominant compound microsatellite markers

@article{Lian2006AnIT,
  title={An improved technique for isolating codominant compound microsatellite markers},
  author={Chunlan L. Lian and Md. Abdul Wadud and Qifang Geng and Kenichiro Shimatani and Taizo Hogetsu},
  journal={Journal of Plant Research},
  year={2006},
  volume={119},
  pages={415-417}
}
An approach for developing codominant polymorphic markers (compound microsatellite (SSR) markers), with substantial time and cost savings, is introduced in this paper. In this technique, fragments flanked by a compound SSR sequence at one end were amplified from the constructed DNA library using compound SSR primer (AC)6(AG)5 or (TC)6(AC)5 and an adaptor primer for the suppression-PCR. A locus-specific primer was designed from the sequence flanking the compound SSR. The primer pairs of the… CONTINUE READING
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