An improved system for gene replacement and xylE fusion analysis in Pseudomonas aeruginosa.

@article{Schweizer1995AnIS,
  title={An improved system for gene replacement and xylE fusion analysis in Pseudomonas aeruginosa.},
  author={Herbert P. Schweizer and Tung T. Hoang},
  journal={Gene},
  year={1995},
  volume={158 1},
  pages={15-22}
}
A novel pUC19-based gene replacement vector has been developed. This vector incorporates (i) the counterselectable sacB marker, (ii) a lacZ alpha allele for blue-white screening, (iii) an oriT for conjugation-mediated plasmid transfer and (iv) unique cloning sites for SmaI and the rare-cutting meganuclease I-SceI. These rare restriction sites are also present on the helper plasmid pUC19Sce. The replacement vector is engineered to contain few restriction sites to gain greater access to… CONTINUE READING
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