An enzymeless biosensor based on CuxO/Cu electrode was investigated in this study. The XRD analysis confirmed that CuxO nanostructured material was composed of Cu2O and CuO. The FESEM images showed that the catalysts were flower-like with large surface area. From cyclic voltammograms, the peak current of CuxO/Cu electrode was 6, 6.3 and 1.7 times higher than that of the Cu foil, Cu2O/GCE and CuO/GCE electrodes, respectively. The biosensor based on CuxO/Cu exhibited excellent performance for glucose detection, giving a linear dependence between current and glucose concentration (R=0.996), with a low detection limit (0.049 mM) and high sensitivity (1.62 mA cm(-2) mM(-1)). Finally, the CuxO/Cu sensor was applied and checked in the glucose determination in blood serum samples.