An improved protocol for the culture of cassava leaf protoplasts

  title={An improved protocol for the culture of cassava leaf protoplasts},
  author={Paul Anthony and Michael R. Davey and J. Brian Power and Kenneth C. Lowe},
  journal={Plant Cell, Tissue and Organ Culture},
Viable protoplasts (yield > 1.9 × 107 g−1 fresh weight; mean viability 85±2%, n=5) were isolated from leaves of axenic shoot cultures of Manihot esculenta Crantz. cv. M. Thai 8. Protoplasts were cultured for up to 50 days in liquid, ammonium-free MS medium, overlaying agarose-solidified B5 medium with short glass rods embedded perpendicularly within, and protruding from, the agarose layer. Control protoplasts were cultured identically, but without glass rods. Sustained protoplast division was… CONTINUE READING

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