Corpus ID: 87206801

An improved protocol for DNA extraction from the faeces of the giant panda

@inproceedings{Zhong2003AnIP,
  title={An improved protocol for DNA extraction from the faeces of the giant panda},
  author={Hua Zhong and Xu-long Lai and Rongping Wei and Zhonglai Liu},
  year={2003}
}
  • Hua Zhong, Xu-long Lai, +1 author Zhonglai Liu
  • Published 2003
  • Biology
  • An improved method that facilitates the extraction of PCR compatible faecal DNA from giant panda's faeces is described. The method involved a novel preprocessing step in DNA extraction. The faeces was washed two or three times with precooled acetone, which removed numerous potential PCR inhibitors, and then digested with proteinase K. The DNA was purified with phenol/chloroform. The faecal DNA obtained was sufficiently pure to support reliable amplification, and was applied as template DNA to… CONTINUE READING

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