An improved protocol for DNA extraction from alkaline soil and sediment samples for constructing metagenomic libraries.

@article{Verma2011AnIP,
  title={An improved protocol for DNA extraction from alkaline soil and sediment samples for constructing metagenomic libraries.},
  author={Digvijay Verma and Tulasi Satyanarayana},
  journal={Applied biochemistry and biotechnology},
  year={2011},
  volume={165 2},
  pages={454-64}
}
An improved single-step protocol has been developed for extracting pure community humic substance-free DNA from alkaline soils and sediments. The method is based on direct cell lysis in the presence of powdered activated charcoal and polyvinylpolypyrrolidone followed by precipitation with polyethyleneglycol and isopropanol. The strategy allows simultaneous… CONTINUE READING