An improved procedure for reconstitution of the uncoupling protein and in-depth analysis of H+/OH- transport.

@article{Winkler1992AnIP,
  title={An improved procedure for reconstitution of the uncoupling protein and in-depth analysis of H+/OH- transport.},
  author={Edith Winkler and Martin Klingenberg},
  journal={European journal of biochemistry},
  year={1992},
  volume={207 1},
  pages={
          135-45
        }
}
An improved procedure for reincorporation of isolated uncoupling protein (UCP) from brown adipose tissue into phospholipid vesicles is reported and H+ uptake in K(+)-driven exchange diffusion quantitatively analyzed. UCP is isolated and reconstituted with medium-length linear-chain alkyl polyoxyethylene. In the critical step of vesicle formation, the stepwise removal of the detergent by polystyrene beads is applied. Vesicles are generated in the presence of solutes and buffers to be… CONTINUE READING
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