An improved plant leaf protein extraction method for high resolution two-dimensional polyacrylamide gel electrophoresis and comparative proteomics.

@article{Alam2013AnIP,
  title={An improved plant leaf protein extraction method for high resolution two-dimensional polyacrylamide gel electrophoresis and comparative proteomics.},
  author={Iftekharul Alam and Sa Sharmin and Kyo-Han Kim and Y-G Kim and Jj Lee and B Lee},
  journal={Biotechnic & histochemistry : official publication of the Biological Stain Commission},
  year={2013},
  volume={88 2},
  pages={61-75}
}
We report here a simple and universally applicable protocol for extracting high quality proteins from plant leaf tissues. The protocol provides improved resolution and reproducibility of two-dimensional polyacrylamide gel electrophoresis (2-DE) and reduces the time required to analyze samples. Partitioning rubisco by polyethylene glycol (PEG) fractionation… CONTINUE READING