An improved microsatellite panel to assess genetic variability of the Italian smooth newt (Lissotriton vulgaris meridionalis)

  title={An improved microsatellite panel to assess genetic variability of the Italian smooth newt (Lissotriton vulgaris meridionalis)},
  author={Vincenzo Buono and Giorgia Galliani and Emiliano Mancini and Francesca Davoli and Chiara Mengoni and Nadia Mucci and Leonardo Vignoli},
  journal={Journal of Genetics},
Habitat loss and fragmentation are threatening amphibians by increasing population isolation. However, artificial waterbodies created for livestock may contrast this phenomenon by providing surrogate habitats for amphibians. Here, we performed a genetic study on an amphibian species, Lissotriton vulgaris meridionalis, in a rural area in central Italy where natural wetlands are disappearing and drinking troughs for cattle watering are widespread. Specifically, we tested a panel of microsatellite… 
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Genetic diversity of Horvath’s Rock Lizard meets current environmental restrictions

It is found that the Horvath’s rock lizard probably survived the Pleistocene glaciations in at least two main refugia, one in the South and the other in the central/northern portion of its distribution.

Can a cow save a newt? The role of cattle drinking troughs in amphibian conservation

It is suggested that drinking troughs represent suitable surrogates for pristine habitats for L. vulgaris meridionalis, suggesting that even man‐made aquatic habitats, associated with traditional husbandry, may help to sustain amphibian populations in landscapes where natural wetlands are scarce.



Characterization of microsatellite loci in the Carpathian newt (Lissotriton montandoni)

Seven polymorphic microsatellite loci were developed for the Carpathian newt and tested for cross-amplification in multiple geographic groups of its sister species L. vulgaris, suggesting that the use of the markers developed in the present study may be limited to geographically restricted groups of the smooth newt.

High genetic diversity of common toad (Bufo bufo) populations under strong natural fragmentation on a Northern archipelago

The results reinforce the notion that spatial and temporal scales of fragmentation need to be considered when studying the interplay between landscape fragmentation and genetic erosion.

New polymorphic microsatellite loci for the Greek smooth newt, Lissotriton vulgaris graecus, and their utility in the nominotypical subspecies

Eight polymorphic microsatellites were isolated from the Greek smooth newt using a microsatellite enrichment protocol and selective hybridization with a biotinylated (AC)11 probe, and the amplification success in the nominotypical subspecies favours the use of these micros Satellite loci in population genetic analyses as well as in the study of contact zones between smooth newT subspecies.

Conservation genetics of amphibians

  • T. Beebee
  • Biology, Environmental Science
  • 2005
Amphibians are good models for investigating the genetics of wild animal populations because they are widely distributed in most ecosystems, easy to sample in breeding assemblages, and amenable to controlled crossings in the laboratory.

Review: microsatellite markers in amphibian conservation genetics

The use of some newly developed analytical tools on newt (Triturus sp.) microsatellite data are demonstrated, practical aspects of using microsatellites for amphibians are discussed, and potential future research directions are outlined.


Itasca populations were significantly different than populations from Nelson County, North Dakota, with FST estimated at about twice the largest pairwise estimates among Itasca samples, however, there were substantial similarities in the overall pattern of allele frequencies at four of the five loci.

Principles for Management of Aquatic-Breeding Amphibians

Coordinated efforts by ecologists and natural resource managers are necessary to balance the conservation of biological diversity with the potential for sustained economic development. Because some

Characterization of microsatellite loci in two closely related Lissotriton newt species

Eight di- and tetranucleotide Lissotriton microsatellite markers developed are suitable for the investigation of population structure, genetic variation and taxonomic identification in the two focal species, and may also be of use in other LissOTriton–Triturus species.

The genetics of amphibian declines: population substructure and molecular differentiation in the Yosemite Toad, Bufo canorus (Anura, Bufonidae) based on single‐strand conformation polymorphism analysis (SSCP) and mitochondrial DNA sequence data

A comprehensive survey of genetic variation across the range of the narrowly distributed Yosemite toad Bufo canorus, a declining amphibian restricted to the Sierra Nevada of California, suggests that management for B. canorus should focus at the individual pond level, and effective management may necessitate reintroductions if local extirpations occur.

Global Amphibian Declines: A Problem in Applied Ecology

The results suggest that most amphibian populations should decrease more often than they increase, due to highly variable recruitment and less variable adult mortality.