An improved method for three-dimensional reconstruction of protein expression patterns in intact mouse and chicken embryos and organs.

@article{AhnfeltRnne2007AnIM,
  title={An improved method for three-dimensional reconstruction of protein expression patterns in intact mouse and chicken embryos and organs.},
  author={Jonas Ahnfelt-R\onne and Mette Christine J\orgensen and Jacob Hald and Ole Dragsbaek Madsen and Palle Serup and Jacob Hecksher-S\orensen},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={2007},
  volume={55 9},
  pages={925-30}
}
We have developed a wholemount immunofluorescence protocol for the simultaneous detection of up to three proteins in mouse and chicken embryos. Combined with Murray's clearing reagent (BABB) and microscope objectives with long working ranges and high numerical apertures mounted on a confocal microscope, cellular resolution can be obtained in depths offering the possibility of examining expression patterns in entire organs or embryos. Three-dimensional projections of the optical confocal… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.
7 Citations
16 References
Similar Papers

References

Publications referenced by this paper.
Showing 1-10 of 16 references

Genetic determinants of pancreatic e - cell development

  • M Jenny, P Collombat, A Mansouri, C Tomasetto, OD Madsen, G Mellitzer
  • Dev Biol
  • 2005

Direct evidence for the pancreatic lineage : NGN 3 1 cells are islet progenitors and are distinct from duct progenitors

  • V Hamburger, HL Hamilton
  • Development
  • 2002

Similar Papers

Loading similar papers…