• Chemistry, Medicine
  • Published in
    Biomedical chromatography…
    2005
  • DOI:10.1002/bmc.479

An improved method for proteomics studies in C. elegans by fluorogenic derivatization, HPLC isolation, enzymatic digestion and liquid chromatography--tandem mass spectrometric identification.

@article{Masuda2005AnIM,
  title={An improved method for proteomics studies in C. elegans by fluorogenic derivatization, HPLC isolation, enzymatic digestion and liquid chromatography--tandem mass spectrometric identification.},
  author={Mayumi Masuda and Hiroshi Saimaru and Norio Takamura and Kazuhiro Imai},
  journal={Biomedical chromatography : BMC},
  year={2005},
  volume={19 7},
  pages={
          556-60
        }
}
An improved method for proteomics studies, which includes the fluorogenic derivertization of protein mixtures with 7-chloro-4-(dimethylaminoethylaminosulfonyl)-2,1,3-benzoxadiazole (DAABD-Cl), followed by HPLC isolation, enzymatic digestion and identification of the derivatized proteins by HPLC-electrospray ionization (ESI)-MS/MS with the probability-based protein identification algorithm, identified 103 proteins in the soluble extract (10 microg protein) of Caenorhabditis elegans.